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KMID : 1161420100130030705
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Journal of Medicinal Food
2010 Volume.13 No. 3 p.705 ~ p.709
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In Vitro Antimicrobial, Antioxidant, and Antispasmodic Activities and the Composition of the Essential Oil of Origanum acutidens (Hand.-Mazz.) Ietswaart
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Goze Ismihan
Alim Ahmet
Cetinus Senay Akkus
Cetin Ali
Durmus Nedim
Atas Ahmet Turan
Vural Nilufer
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Abstract
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The radical scavenging, antimicrobial, and antispasmodic activities and the composition of Origanum acutidens (Hand.-Mazz.) Ietswaart (Order Labiatte) essential oil were evaluated in vitro. Gas chromatography/mass spectrometry analysis of the oil resulted in the identification of 20 compounds, representing 93.61% of the oil; carvacrol (65%) was the main component. The sample was also subjected to a screening for antioxidant activity by using the 1,1-diphenyl-2-picrylhydrazyl and β-carotene/linoleic acid assays. The essential oil exhibited strong antioxidant activity. Using the agar disk diffusion method, antimicrobial activities of the essential oils were determined for nine microorganisms; antimicrobial activity was shown against eight of them. O. acutidens oil exhibited spontaneous contractions in rat ileum at the 100% level at 0.1?mg/mL level.
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KEYWORD
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antimicrobial activity, antioxidant activity, antispasmodic activity, essential oil, Origanum acutidens
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